Search results for "Protein storage vacuole"
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Distinct lytic vacuolar compartments are embedded inside the protein storage vacuole of dry and germinating Arabidopsis thaliana seeds.
2011
International audience; Plant cell vacuoles are diverse and dynamic structures. In particular, during seed germination, the protein storage vacuoles are rapidly replaced by a central lytic vacuole enabling rapid elongation of embryo cells. In this study, we investigate the dynamic remodeling of vacuolar compartments during Arabidopsis seed germination using immunocytochemistry with antibodies against tonoplast intrinsic protein (TIP) isoforms as well as proteins involved in nutrient mobilization and vacuolar acidification. Our results confirm the existence of a lytic compartment embedded in the protein storage vacuole of dry seeds, decorated by γ-TIP, the vacuolar proton pumping pyrophospha…
The Formation of The Plant Vacuolar System
1999
The plant vacuole is a multifunctional subcellular compartment. In general, the vacuolar system is constituted of a single or few vacuoles. About 80% of the volume of mature plant cells is occupied by vacuoles. These organelles are limited by a single membrane, the tonoplast. According to the cell type and to particular functions, the vacuolar content can vary from a homogeneous consistency to a heterogeneous matrix containing crystalloid structures (Boiler, Wiemken, 1986; Marty et al., 1980). From the biotechnological point of view, the endomembrane system of plants, particularly the ER and vacuoles, can be envisioned as the ultimate environment for stable protein accumulation. Several rep…